Cloning of the gene encoding human recombinant blood \u2013 coagulation factor WIII

Background: Hemophilia A is a genetic bleeding disorder that results from a deficiency in factor VIII. The prevalence of Hemophilia in Vietnam is rather high (2/34830 people) and Vietnam has high usage demand for factor VIII in the treatment and prevention of the disease. Therefore, it is necessary to study and produce recombinant blood \u2013 coagulation factor WIII. Objective: To clone successfully A1A2 and A3C2 gen fragment encoding factor VIII. Subject and Method: Amplify A1A2 and A3C2 gene fragments by PCR from human cDNA. PCR products were ligated into cloning vector pQE \u2013 30UA. Recombinant plasmids were transformed into E.coli DH5 alpha host strain. Inserted A1A2 and A3C2 gene fragments were checked by PCR and restriction enzymes. Result: Successfully amplifying functional gene fragments encoding factor VIII using specific primers. Conclusion: Obtaining pQE \u2013 30UA vector carrying A1A2 and A3C2 fragments encoding factor VIII. This is the premise result for the next studies on synthesis of recombinant factor WIII and application of genetic therapy.

Generate and purify antibody against human Heparansulphate interacting protein (hHIp) in rabbit

Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'

HLA-DR typing by PCR-SSP technique

Participants of this study are 11 healthy people. Method of amplification for a specific gene fragment in ADN was used. Sample 1 was controlled by PCR-SSO technique in National Laboratory for Histointegration of Saint-Luis Hospital, Paris. Sample 11 was controlled by PCR-RFLP in Immunological Laboratory of Department for Microbiology and Immunology, Aichi University, Japan. Result of control was similar to that of PCR-SSP technique.

HLA-DR typing by PCR-SSP technique

Participants of this study were 11 healthy people. Method of amplification for a specific gene fragment in ADN was used. Sample 1 was controlled by PCR-SSO technique in National Laboratory for Histointegration of Saint-Luis Hospital, Paris. Sample 11 was verified by PCR-RFLP in Immunological Laboratory of Department for Microbiology and Immunology, Aichi University, Japan. Result of verification was similar to that of PCR-SSP technique.